Summary:
Purpose: To assess the bacterial extrusion by using manual, multiple-file continuous rotary system (ProTaper) and single-file continuous rotary system (One Shape).
N= 42 human mandibular premolars were inoculated with Enterococcus faecalis by using a bacterial extrusion model.
Materials/Methods:
- The teeth were divided into 3 experimental groups (n = 12) and 1 control group (n = 6).
- The root canals of experimental groups were instrumented according to the manufacturers’ instructions by using manual technique, ProTaper rotary system, or One Shape rotary system.
- Test apparatus Fig 1
- Sterilized saline was used as an irrigant, and bacterial extrusion was quantified as colony-forming units/milliliter.
- The results obtained were statistically analyzed by using one-way analysis of variance for inter-group comparison and post hoc Tukey test for pair-wise comparison.
Most highlighted Results:
- All the instrumentation techniques resulted in bacterial extrusion, with manual step-back technique exhibiting significantly more bacterial extrusion than the engine-driven systems.
- Of the 2 engine-driven systems, ProTaper rotary extruded significantly more bacteria than One Shape rotary system.
- Table 1
Clinical significance:
The engine-driven nickel-titanium systems were associated with less apical extrusion. The instrument design may play a role in amount of extrusion.
