Purpose: to determine the effect of demineralization of resected root ends on the temporal and qualitative healing on the dentoalveolar and alveolar tissues in excisional wound site during periradicular surgery.
N= 24 (Mand. 3rd and 4th premolars of 6 dogs).
Materials and method:
1.NSRCT for all premolars
2. Triangular full mucoperiosteal flaps elevated, root end resected.
3. Experimental group (demeneralized): burnished with 50% citric acid for 2 min., Control: no treatment.
4.Surgical flaps were reapproximated, compressed and sutured.
5.Animals were placed on soft diets and administered ibuprofen.
6.Specimen coded blocks were later trimmed and placed in 0.5 EDTA for decalcification.
7. Light microscopic evaluation of specimens was based on: :
– clotting & inflammation 8.
– Epithelial healing
Most highlighted results:
•In experimental gp: cementum deposition was shown at 12 days, at 16 days cementum covered more than 50% of resected roots surface areas, dentoalveolar healing with re-formation apical attachment after 45 days. •In control gp: no cementum was seen until day 16 and at 45 days cementum covered less than 50%.
•Non-demeneralized root ends were associated with delayed and irregular pattern of cementogenesis. While, early, evenly distributed cementogenesis was seen with demeniralized root ends.