Summary:
Purpose: To compare the antimicrobial effects of 2.5% sodium hypochlorite (NaOCl) and 0.12% chlorhexidine digluconate (CHX) when used as irrigants during treatment of teeth with apical periodontitis
Materials/Methods:
n: 47 single rooted/canal teeth with necrotic pulp and asymptomatic apical periodontitis
Inclusion & Exclusion:
were stated Bacterial samples were taken at baseline S1 (filing with #15 k using saline only), after chemomechanical preparation with NaOC S2L n: 30, and after chemomechanical preparation with 0.12% CHX S2 n: 17. Using PCR and reverse capture checkboard DNA-DNA hybridization
Highlights:
-S1 yielded positive PCR results for bacteria.
-In the 2.5% NaOCl group 40% were PCR negative for bacterial presence.
-In the CHX group 47% had a negative PCR results for bacteria.
-No significant difference was observed when comparing the incidence of negative PCR results in S2 samples from NaOCl and CHX groups
-Significant difference in the bacterial presence between S1 and S2 of both groups (NaOCL and CHX).
Conclusion:
Using either NaOCl or CHX as the irrigant significantly reduced the number of bacterial taxa and their levels in infected root canals, with no significant difference between these 2 irrigants.
Combining NaOCL and CHX is recommended to benefit from the substantivity of CHX to dentin and organic matter dissolution from NaOCL.