Comparison of the In Vivo Antimicrobial Effectiveness of Sodium Hypochlorite and Chlorhexidine Used as Root Canal Irrigants: A Molecular Microbiology Study

By Rôças IN, Siqueira JF Jr

Date: 07/2012
Journal: JOE

Summary:  

Purpose: To compare the antimicrobial effects of 2.5% sodium hypochlorite (NaOCl) and 0.12% chlorhexidine digluconate (CHX) when used as irrigants during treatment of teeth with apical periodontitis

Materials/Methods: 

n: 47 single rooted/canal teeth with necrotic pulp and asymptomatic apical periodontitis

Inclusion & Exclusion:

were stated Bacterial samples were taken at baseline S1 (filing with #15 k using saline only), after chemomechanical preparation with NaOC S2L n: 30, and after chemomechanical preparation with 0.12% CHX S2 n: 17. Using PCR and reverse capture checkboard DNA-DNA hybridization

Highlights:

-S1 yielded positive PCR results for bacteria.

-In the 2.5% NaOCl group 40% were PCR negative for bacterial presence.

-In the CHX group 47% had a negative PCR results for bacteria.

-No significant difference was observed when comparing the incidence of negative PCR results in S2 samples from NaOCl and CHX groups

-Significant difference in the bacterial presence between S1 and S2 of both groups (NaOCL and CHX).

Conclusion:

Using either NaOCl or CHX as the irrigant significantly reduced the number of bacterial taxa and their levels in infected root canals, with no significant difference between these 2 irrigants.

Combining NaOCL and CHX is recommended to benefit from the substantivity of CHX to dentin and organic matter dissolution from NaOCL.