Interactions of ethylenediamine tetraacetic acid with sodium hypochlorite in aqueous solutions Journal, year: International Endodontic Journal,2003

By Grawehr M, Sener B, Waltimo T, Zehnder M.

Date: 08/2004
Journal: JOE


Purpose: The goal of the present study was to evaluate effect of mixing the EDTA and NaOCl solutions.

N= was not mentioned clearly


•Solutions consisting of 8.5% EDTA,  0.5% NaOCl and a mixture of 17% EDTA/1% NaOCl

•Amounts of available chlorine were determined in the solutions

•Calcium chelation capacity was titrated with a pure calcium solution using an indicator

•Antimicrobial potential :recorded using an agar diffusion test in plates incubated with E- faecalis or Candida albicans.

•8 specimens obtained from pigs mucosa were incubated with test solutions, evaluated at the start of the incubation and after 15,30,60,90 and120 min exposure to the solution for tissue dissolution

Most highlighted Results:

•Presence of NaOCl had little effect on the calcium-chelating ability or on the antimicrobial efficacy of EDTA.

•chlorine content is less in the combined EDTA/NaOCl solution(0.06%) compared to NaOCl mixture with deionized water (0.50%).

•The EDTA/NaOCl solution did not dissolve more tissue than an equivalent pure EDTA solution at any time (Fig1 ). •Antimicrobial effect (table1)

Clinical significance:

 EDTA has -ve effect on tissue dissolving capacity and antimicrobial efficacy of NaOCl; when combined copious irrigation with NaOCl should be done after.