Ultrasonic disinfection of the root canal

By Martin H

Date: 01/1976
Journal: OOO


Purpose: To quantify the bactericidal efficiency of ultrasonics in the root canal and as a component with potentiated

acid 1% of 1,5 pentanedial (Glutaraldehyde) and 5.5% NaOCl as an endodontic irrigants.


3 experimental Gps n: 16 Max and Mand Molars (

Gp.1; ultrasonic with a neutral buffer;

Gp.2: ultrasonic with glutaraldehyde and NaOCL;

Gp.3: ultrasonic with glutaraldehyde and NaOCL in the presence of organic horse serum)

-The tested bacteria were: Streptococcus faecalis; Streptococcus mitis; Staphylococcus aureus, and E.coli

-The ultrasonic irradiator used was titanium tip (I/‘8 inch in diameter) driven by a lead zirconated sandwich transducer.

– Teeth were prepared using telescopic filing technique (Step Back).  After mechanical preparation, teeth were cleaned ultrasonically for 5 minutes. The apices were then sealed with Cavit and Cold-curing acrylic molds were placed around all roots to the CEJ. The prepared teeth were autoclaved for 30 m. then bacteria was inoculated in to the pulp chamber.


-In Gp.1 bactericidal effect was noted. Thus, ultrasound have a bactericidal effect because of its physical biologic acoustic action. It plateau’s after 4-5 minutes Table.I.

-The addition of bactericidal agents in Gp.2 enhanced the effective destruction of the bacteria Table.II.

–      In Gp.3 the presence of serum 1% Glutaraldehyde was slightly more effective than NaOCL in the immediate

reducing action on the organisms Table.III.


Ultrasonics alone had a reducing effect on but coupling it with a biocidal agent led to a more efficient bactericidal synergism.