In vitro microbial leakage of endodontically treated teeth using new and standard obturation techniques

By Shipper G, Trope M

Date: 12/2004
Journal: JOE


Purpose: To evaluate and compare the ability of bacteria to penetrate new and conventional obturation techniques and materials

N= 140 single rooted extracted teeth


•The crowns were removed and the canals were instrumented up to an ISO size of #40 or #50

•The teeth were divided into 10 groups of 15 teeth for the experimental (table1)

•All the groups were instrumented with Profile 04 instruments except G6 & G8. (Light speed).

•Split chamber microbial leakage model was used in which Streptococcus mutans  placed in the upper chamber could reach the lower chamber only through the obturated canal.

•Leakage was assessed every day for 30 days

Most highlighted Results:

•The SimpliFill-FibreFill group (group 8) proved to have the best obturation technique and was statistically significantly superior when compared with any of the other experimental groups.

•Both FibreFill and SimpliFill were statistically superior to the conventional obturation techniques.

•Microbial leakage occurred more quickly in lateral and vertical condensation techniques compared with SimpliFill and FibreFill techniques.

Clinical significance:

Combination of SimpleFill and FiberFill techniques provided superior resistance to leakage.