Reaction of rat connective tissue to polyethylene tube implant, part I​

By Torneck CD.

Date: 11/1967
Journal: Oral Surg Oral Med Oral Patho

Summary:  ​

Purpose:  to investigate the influence of an unfilled space and other local factors normally associated with endodontic repair on the healing of connective tissue. ​

N= 24 implants  implanted in 6 rats.​


  • Varying lengths (4, 6, 10 mm) and diameters (0.58- 0.1.4mm) of polyethylene tubing were surgically implanted into the dorsal subcutaneous tissues of rats.​
  • half of the tubes were open at both ends, the other half had one end sealed. The tubes were left implanted for a period of 60 days, following which the animals were killed and the implants, together with the surrounding tissue, were excised.​
  • Specimens were then fixed in 10% formalin and sectioned serially and stained with hematosylin and eosin stain​

Most highlighted Results:  ​

  • After 60 days the tubes were  surrounded by a non-inflamed connective tissue capsule.​
  • in the open tubes: ingrowth of connective tissue occurred throughout the length of tubes of shorter lengths and larger diameters.​
  • In the closed tubes, the ingrowth was observed in the upper 0.25 to 0.5 mm. this invagination occurred primarily in tubes of the smaller diameters.​

Clinical significance:

 The over-all findings of the study indicated that under-filling a root canal which has been thoroughly and completely cleaned and adequately disinfected would probably result in healing of the surrounding peri-apical tissue.​