Reaction of rat connective tissue to polyethylene tube implant :part II

By Torneck CD.

Date: 10/1967
Journal: Oral Surg Oral Med Oral Patho

Summary:  ​

Purpose:  to investigate  the reaction of the connective tissue to filling of the tubes with sterile and contaminated debris ​

N= 24 implants  implanted in 6 animals.​


  • Varying lengths (4, 6, 10 mm) and diameters (0.58- 0.1.4mm) of polyethylene tubing” were surgically implanted into the dorsal subcutaneous tissues of rats. Tubes were sealed at one end disinfected.​
  • In the 1st part of the study: the Lumina of the tubes were filled with sterile fragments of muscle tissue​
  • In the 2nd part of the study: the muscles was autoclaved and then inoculated with gram negative cocci. ​
  • The tubes were left implanted for a period of 60 days, following which the animals were killed and the implants, together with the surrounding tissue, were excised.​
  • Specimens were then fixed in 10% formalin and later sectioned serially and stained with hematoxylin and  eosin or Mallory’s collagen stain​

Most highlighted Results:  ​

  • In the 1st part:  tubes were surrounded by a non-inflamed connective tissue capsule except in the area directly opposite the orifice of the tube lumen. (mild to moderate inflammation).​
  • In 2nd part: connective tissue showed moderate to intense inflammation and abscess formation​

Clinical significance:  

prognosis for repair about the open end of the tube was least favorable when​ the lumen was filled with muscle debris and microorganisms. Both groups produced conditions that were less favorable for repair than when the lumen of the tube was sterile and clean. ​