Studies of Sargenti’s technique of endodontic treatment: short-term response in monkeys.

By Cohler CM, Newton CW, Patterson SS, Kafrawy AH.

Date: 09/1981
Journal: JOE

Purpose: To evaluate the response to the Sargenti technique and filling material, RC-2B, at six months and a year

     after treatment.

According to Sargenti: Essentials of the N2 method include speed, simplicity, use of a special formula (N2 or RC-2B) as a canal filler, no use of the rubber dam, and engine-powered canal preparation.

N2: Formaldehyde containing ZOE.

RC-2B: American counterpart of N2.


-Three young adult Macaca speciose monkeys with healthy permanent dentition.

-Initial radiographs were taken, no evidence of any pathologic conditions.

-Pulpal and periapical pathoses were then induced.

-Monkeys 1 and 2 were treated for either pulpitis or necrosis and monkey 3 was treated for both.


– Access, instrumentation with peeso reamer to full length (confirmed by RGs)

                –  No irrigation was used, RC-2B mixed to creamy consistency and delivered to canals by lentulo spiral.

                – Restored with cotton pellet and amalgam, final RGs.

-Animals were periodically examined clinically and radiographitally every three months until they were sacrificed.

– Monkey 3 was killed at six months, whereas 1 and 2 were killed at a year.

-Samples processing and histological analysis.

Most highlighted Results:

•Periapical pathosis was evident in association with all teeth treated for puIpitis at six months and a year after treatment.

•A comparison with the findings a month after treatment showed that the periapical lesions that developed were more severe at the longer observation periods.

•Zones of fixed pulp tissue were encountered in most of the teeth treated for pulpitis at six months but were less evident a year after treatment.

•In teeth with induced necrosis, both the treated and untreated control teeth showed basically the same periapical


Clinical significance:

– Proved principles of endodontics should not be violated.

– Closer attention should be paid to pulpal debris and microorganisms in the canal, as well as to obtaining a sealed foramen to allow the body’s defenses to overcome any residual infection and to permit healing to occur.