Histological study of furcation perforations treated with Tricalcium phosphate, hydroxylapatite, amalgam, and Life

By Balla R, LoMonaco CJ, Skribner J, Lin LM.

Date: 12/1991
Journal: JOE



To examine histologically the tissue response to experimental furcation perforations immediately treated with tricalcium phosphate and hydroxylapatite, and to compare the results with those treated with amalgam and Life.

Materials and Methods:

 – n: 120 teeth (max and mandibular Ms & pms) in 6 rhesus monkeys

-Teeth were accessed, instrumented, and perforated in the floor of the chamber using round bur #4. Orifices were then sealed with Amalgam in all gps and after perforation repair teeth were restored with amalgam in all gps except  +ve CG.

-Teeth were divided in to 4 experimental gps in regards to the repairing material Gp.1 Tricalcium phosphate: 30 teeth, Gp.2 Hydroxylapatite: 30 teeth, GP.3 LifeTM (CaOH): 24 teeth, Gp.4 Amalgam: 24 teeth, and +ve CG: 12 teeth.

-The animals were killed two at a time at intervals of 2, 4, and 6 months after the experiment.

-Mandibles were sectioned, fixed, serial sectioned, and stained with H&E and Brown and Brinn stain.he

-Tissue inflammatory responses were classified as: none; mild; moderate; and severe.

Most highlighted results:

-Intensity of inflammatory response is shown in Fig.1

-The intensity of inflammation tended to decrease as the experimental period increased except in positive control.

-Control Group: heavy infiltrate of inflammatory cells, epithelium formation , granulation tissue & Gram +ve bacteria.

-Amalgam, Life, and hydroxylapatite seemed to cause a lesser degree of inflammation when compared with tricalcium phosphate at the 6-month experimental period.

-Epithelium and bone resorption were observed more frequently in the +ve CG as shown in Table.1.

-Non of the experimental gps showed complete healing or new bone formation at the site of perforation.

Clinical Significance:

-Amalgam, Life, and hydroxylapatite seemed to cause a lesser degree of inflammation than tricalcium phosphate.