Perforation repair comparing mineral trioxide aggregate and amalgam using an anaerobic bacterial leakage model

By Nakata TT, Bae KS, Baumgartner JC

Date: 02/2000
Journal: JOE


Purpose: to compare the sealing ability of MTA and amalgam in furcal perforations of extracted human molars using an anaerobic bacterial leakage model

N= 42 extracted human maxillary and mandibular molars


•Furcal perforations prepared using a high-speed bur from the external surface

•Access cavities were prepared, and all the teeth surfaced were coated with 2 layers of nail polish

•2 experimental groups of 18 according to the repair material (1: MTA  2: amalgam)

•3 teeth that were perforated but not repaired (+ve control),  3 teeth not perforated (-ve control)

•After perforation repair, A dual chamber anaerobic bacterial leakage model was assembled

•Upper chamber contained culture broth with Fusobacterium nucleatum ,lower chamber contained sterile bacterial broth

•The samples were observed every 2 to 3 days for turbidity and/or color change of the broth, indicating bacterial growth from penetration of bacteria past the perforation repair

•The experimental period last for 45 days.

Most highlighted Results:

•8 of 18 amalgam samples leaked (44%) , whereas none of the 18 MTA samples leaked

•MTA was significantly better than amalgam in preventing leakage of furcal perforation repairs

Clinical significance:

MTA was superior to amalgam as a perforation repair material