Summary:
Purpose: to compare the sealing ability of MTA and amalgam in furcal perforations of extracted human molars using an anaerobic bacterial leakage model
N= 42 extracted human maxillary and mandibular molars
Materials/Methods:
•Furcal perforations prepared using a high-speed bur from the external surface
•Access cavities were prepared, and all the teeth surfaced were coated with 2 layers of nail polish
•2 experimental groups of 18 according to the repair material (1: MTA 2: amalgam)
•3 teeth that were perforated but not repaired (+ve control), 3 teeth not perforated (-ve control)
•After perforation repair, A dual chamber anaerobic bacterial leakage model was assembled
•Upper chamber contained culture broth with Fusobacterium nucleatum ,lower chamber contained sterile bacterial broth
•The samples were observed every 2 to 3 days for turbidity and/or color change of the broth, indicating bacterial growth from penetration of bacteria past the perforation repair
•The experimental period last for 45 days.
Most highlighted Results:
•8 of 18 amalgam samples leaked (44%) , whereas none of the 18 MTA samples leaked
•MTA was significantly better than amalgam in preventing leakage of furcal perforation repairs
Clinical significance:
MTA was superior to amalgam as a perforation repair material