Purpose: to compare the ability of two types of MTA (Gray & off-white) for sealing furcation perforations in extracted human molars using an anaerobic, bacterial-leakage model.
N= 40 extracted mandibular and maxillary molars.
•Difference between gray and off-white MTA: the off-white-colored formula lacks the tetracalcium aluminoferrite. The lack of this iron-containing compound may account for its off-white appearance.
•Both types are 75% Portland cement, 20% bismuth oxide, and 5% gypsum by weight
a) Experimental group 1 (n=18): Perforation repaired with gray-colored MTA
b)Experimental group 2 (n=18) Perforation repaired with off-white-colored MTA.
c)Positive control (n=2) Perforation made but not repaired
d)Negative control (n=2) No perforations
•Standardized access cavities were prepared, and all the teeth surfaced were coated with 2 layers of nail polish.
•A high-speed bur was used to prepare furcal perforations.
•Teeth were randomly divided into the experimental groups based on the repair material.
•After perforation repair, A dual chamber anaerobic bacterial leakage model was assembled.
•Upper chamber contained the culture broth with Fusobacterium nucleatum, lower chamber contained sterile bacterial broth.
•The samples were observed every 2 to 3 days for turbidity of the broth, indicating bacterial growth from penetration of bacteria past the perforation repair.
•The experimental period last for 60 days.
Most highlighted Results:
•Between 15 and 21 days:
•(11%) gray-colored MTA samples leaked and
•(16%) off-white-colored MTA samples leaked
•There was no significant difference between the two types of MTA in preventing leakage of F. nucleatum past furcal perforation repairs.
Conclusion: White MTA performed similarly to gray MTA in perforation repair.