Purpose: to determine if Enterococcus spp. are more prevalent in endodontically treated teeth with periradicular lesions compared with teeth that require retreatment but have no periradicular rarefaction.
N= 58, in which 22 cases had no periradicular lesions and 36 were with lesions.
•Patients had an endodontically treated tooth requiring endodontic retreatment (Group 1) with radiographic evidence of periradicular periodontitis; (Group 2) without apical pathosis.
• RCT completed at least 1 yr ago.
•The following variables were registered for each patient:
a) Peri-operative S&S b) Quality of previous root filling;
c) Quality of the coronal restoration; and d) History of diabetes mellitus.
•Rubber dam, access and disinfection with 30% H2O2 and 5% iodine tincture and 6% NaOCl.
•Sodium hypochlorite was neutralized with 5% sodium thiosulphate. a surface specimen was collected with paper points
•GP removed with no slovent, apical size reached #25, saline, specimens were obtained using paper points.s
•DNA extraction and PCR amplification were performed using ubiquitous 16S rDNA bacterial primers, as well as Enterococcus spp.-specific primers.
•Most highlighted Results:
1.Bacterial detection (89.7%) , Enterococcus spp. was present in only 12.1%. 2.Teeth with a PA lesion were significantly more likely to have bacteria than teeth without a periradicular lesion.
3.There was a statistically significant inverse relationship between presence of Enterococcus and lesion status. Enterococcus spp. was associated with non-lesion teeth.
Enterococcus spp. are significantly associated with endodontic failure but enterococci are not associated with disease.