An in vitro comparison of bacterial leakage of three common restorative materials used as an intracoronal barrier

By Fathi B, Bahcall J, Maki JS.

Date: 09/2007
Journal: JOE

Summary: 

Purpose: to evaluate quantitatively the effectiveness of three different restorative materials used as an intracoronal barrier to prevent micro-leakage of endodontically treated teeth

N= 55 extracted single-canal teeth

Materials/Methods:

•Teeth decoronated to the CEJ then prepared and obturated using warm vertical compaction

•15 teeth were randomly assigned in each expiremental groups (2 mm of intracroronal barrier)

-Group 1 >  Ketac-Cem (permanent glass ionomer cement)

-Group 2 > Clearfill Protect Bond/Clearfil AP-X  (a self-etching adhesive system and light-cure composite) ØGroup 3 > Maxcem (a self-etching/self-adhering resin cement)

-Positive control  >  5 teeth were obturated, no coronal restorations, inoculated with E.faecalis

-Negative control  >  5 teeth were obturated, no coronal restorations, inoculated with sterile BHI broth

•Root surfaces coated with two layers of nail polish, except the coronal surface and the apical 2 mm

•Microleakage tested using a two-chamber bacterial method, and E.faecalis used as a microbial marker

•Samples were incubated aerobically at 37c for 120 days

•Bacterial leakage was determined by change in the turbidity in the medium

Most highlighted Results:

•All positive controls leaked within 60 days •Negative control broth remained clear throughout the entire experimental period

•The presence of E.faecalis in all the samples that turned turbid

•No significant difference in bacterial leakage was found between the 3 groups tested

Clinical significance:

The placement of an intra-coronal barrier will improve the coronal seal