Human saliva coronal microleakage in obturated root canals: an in vitro study.

By Magura ME, Kafrawy AH, Brown CE, Newton CW

Date: 10/1991
Journal: JOE

Purpose:  to evaluate coronal leakage of human saliva in endodontically treated maxillary anterior teeth. .

N= 160 maxillary anteriors


•The teeth were instrumented to size 60 H file.

•specimens distribution (Fig.1):

•10 teeth were not obturated; (+ve controls)

•100 teeth were obturated by lateral condensation of GP and Roth’s sealer but did not receive temporary restoration (experimental group)

•50 teeth were obturated and received IRM temporaries to a thickness of 3 mm. ( -ve  controls).

•All teeth were immersed in 50 ml of whole human saliva and kept at 37 degrees and 100% humidity.

•At 2, 7, 14, 28, and 90 days, 32 teeth were removed from the saliva (2 +ve controls, 10 –ve controls, 20 experimental).

•Evaluation of saliva leakage:

•Bacterial culture was done for the 2 positive controls.

•10 experimental and 10 – controls subjected to histologic examinations. •10 experimental were immersed in Pelikan dye and assessed for dye penetration.

Most highlighted Results: 

•Salivary penetration assessed in the H & E-stained sections was significantly less than the dye penetration.

•Salivary penetration at 3 months was significantly greater than at the four earlier study periods. No difference among other time periods

•Leakage for the experimental group of teeth with no IRM restorations was not significantly different from the leakage in the control teeth with intact IRM restorations.

Clinical significance:

The results suggest retreatment of obturated root canals that have been exposed to the oral cavity for at least 3 months.