- To examine electrospun scaffolds containing antibiotics and determine fiber morphology, chemical structure, mechanical properties, and drug release.
- N= N/A
- Exclusion criteria: N/A
- Inclusion criteria: N/A
- Positive/negative control: Polydioxanone monofilament suture material (PDS) w/no antibiotic added
- Control & Design: Metronidazole or Ciprofloxacin added to PDS in concentrations of 5%, 25%.
- Drug release: Determined w/high-pressure liquid chromatograph equipped w/UV-vis detector
- Antimicrobial testing: Evaluated against P. gingivalis/E. faecalis w/biofilm and agar diffusion assays. Automated colony counter quantified CFU’s and SEM evaluated biofilm inhibition qualitatively.
- Statistical analyses: One-way ANOVAs compared groups for differences in fiber diameter. Two-way ANOVAs compared differences in tensile strengths/modulus.
- Criteria of evaluation: Bacterial inhibition, drug release, mechanical properties.
- CIP incorporation (gyrase inhibitor), led to significant inhibition in growth of both Ef and Pg.
- MET at both concentrations did not lead to reduction in Ef growth, but inhibited Pg.
- Concentrations used are significantly lower than currently used for TAP/DAP. (5 wt% CIP = 280 μg, and 25 wt% CIP = 1 mg) vs. 1g/mL
- CIP incorporation into scaffold showed significantly less strength in comparison to MET
Antibiotic-containing scaffolds hold promise for the improvement of current regenerative strategies by providing a drug delivery system to disinfect necrotic immature permanent teeth through a controllable release of low, yet effective, antibiotic doses.