Bioactive nanofibrous scaffolds for regenerative endodontics.

By Bottino MC, Kamocki K, Yassen GH, Platt JA, Vail MM, Ehrlich Y, Spolnik KJ, Gregory RL.

Date: 01/2013
Journal: JDR

Purpose:

  • To examine electrospun scaffolds containing antibiotics and determine fiber morphology, chemical structure, mechanical properties, and drug release.

Materials/Methods:

  • N= N/A
  • Exclusion criteria: N/A
  • Inclusion criteria: N/A
  • Positive/negative control: Polydioxanone monofilament suture material (PDS) w/no antibiotic added
  • Control & Design: Metronidazole or Ciprofloxacin added to PDS in concentrations of 5%, 25%.
  • Drug release: Determined w/high-pressure liquid chromatograph equipped w/UV-vis detector
  • Antimicrobial testing: Evaluated against P. gingivalis/E. faecalis w/biofilm and agar diffusion assays. Automated colony counter quantified CFU’s and SEM evaluated biofilm inhibition qualitatively.
  • Statistical analyses: One-way ANOVAs compared groups for differences in fiber diameter. Two-way ANOVAs compared differences in tensile strengths/modulus.
  • Criteria of evaluation: Bacterial inhibition, drug release, mechanical properties.

Results:

  • CIP incorporation (gyrase inhibitor), led to significant inhibition in growth of both Ef and Pg.
  • MET at both concentrations did not lead to reduction in Ef growth, but inhibited Pg.
  • Concentrations used are significantly lower than currently used for TAP/DAP. (5 wt% CIP = 280 μg, and 25 wt% CIP = 1 mg) vs. 1g/mL
  • CIP incorporation into scaffold showed significantly less strength in comparison to MET

Clinical Significance:

Antibiotic-containing scaffolds hold promise for the improvement of current regenerative strategies by providing a drug delivery system to disinfect necrotic immature permanent teeth through a controllable release of low, yet effective, antibiotic doses.