Clinical, Radiographic, and Histological Observation of a Human Immature Permanent Tooth with Chronic Apical Abscess after Revitalization Treatment

By Shimizu E, Ricucci D, Albert J, Alobaid AS, Gibbs JL, Huang GT, Lin LM.

Date: 01/2013
Journal: JOE


  • Examine the histology of a revitalization case


  • 9 year old male patient had immature #9 with traumatic, complicated crown fracture with development of chronic apical abscess
  • Revitalization done with 2.6% NaOCl, CaOH as an intracanal medication for 11 days then bleeding induced and MTA placed with composite restoration
  • After 26 months tooth had a cervical horizontal fracture. Extracted and prepared for histological and immunohistochemical examination


  • Clinically and radiographically, the revitalization was successful because of the absence of signs and symptoms and the resolution of periapical lesion as well as thickening of the canal walls and continued root development.
  • The tissue formed in the canal was well-mineralized cementum- or bone-like tissue identified by routine histology and immunohistochemistry (no pulp-like tissues)

Clinical Significance:

Tissue formed in the canal during revascularization was well-mineralized cementum- or bone-like tissue