- Investigate the association of CD14 -260C>T and TLR4 +896A>G gene polymorphisms with post-treatment apical periodontitis in Brazilian individuals.
- N= 41 patients with post-treatment apical periodontitis and 42 individuals with root canal–treated teeth exhibiting healed/healing periradiculartissues (controls).
- Inclusion criteria: 1 tooth with endodontic treatment ranked as adequate. Treated teeth had coronal restorations
- ranked as adequate. All patients from Rio de Janeiro.
- Exclusion criteria: No RCT, not adequate RCT/coronal restoration.
- Positive/negative control: Patients who received RCT and exhibited healing periapical tissues.
- Design: Saliva was collected from the participants; DNA was extracted and used for CD14 and TLR4 genotyping using the polymerase chain reaction–restriction fragment length polymorphism approach and a real-time PCR TaqMan assay.
- Criteria of evaluation: Periapicalhealing, TLR4/CD14 genotype differences
- No specific genotype or allele of the CD14 and TLR4 genes was positively associated with post-treatment apical periodontitis (P > .05).
Data from the study suggests that polymorphisms in the CD14 and TLR4 genes do not influence the response to endodontic treatment of teeth with apical periodontitis.