Polymorphism of the CD14 and TLR4 genes and post-treatment apical periodontitis.

By Rôças IN, Siqueira JF Jr, Del Aguila CA, Provenzano JC, Guilherme BP, Gonçalves LS.

Date: 01/2014
Journal: JOE


  • Investigate the association of CD14 -260C>T and TLR4 +896A>G gene polymorphisms with post-treatment apical periodontitis in Brazilian individuals.


  • N= 41 patients with post-treatment apical periodontitis and 42 individuals with root canal–treated teeth exhibiting healed/healing periradiculartissues (controls).
  • Inclusion criteria: 1 tooth with endodontic treatment ranked as adequate. Treated teeth had coronal restorations
  • ranked as adequate. All patients from Rio de Janeiro.
  • Exclusion criteria: No RCT, not adequate RCT/coronal restoration.
  • Positive/negative control: Patients who received RCT and exhibited healing periapical tissues.
  • Design: Saliva was collected from the participants; DNA was extracted and used for CD14 and TLR4 genotyping using the polymerase chain reaction–restriction fragment length polymorphism approach and a real-time PCR TaqMan assay.
  • Criteria of evaluation: Periapicalhealing, TLR4/CD14 genotype differences


  • No specific genotype or allele of the CD14 and TLR4 genes was positively associated with post-treatment apical periodontitis (P > .05).

Clinical Significance:

Data from the study suggests that polymorphisms in the CD14 and TLR4 genes do not influence the response to endodontic treatment of teeth with apical periodontitis.