- Measure the minimum apical diameter for regeneration to occur.
- Subjects: single-rooted mature beagle teeth
- N= 15
- Experimental Design: extraction -> wire-cutter apicoectomy -> photographed -> replanted for 90 days.
- Sectioned, stained, and evaluated under light microscope.
- Criteria of evaluation:the amount of vital tissue filling the pulp chamber 90 days after replantation, 4 different categories were distinguished:
- 1. More than two thirds of the pulp chamber filled
- 2. Between one third and two thirds of the pulp chamber filled
- 3. Less than one third of the pulp chamber filled (apical area only)
- 4. No ingrowth
- No teeth showed no ingrowth
- The new vital tissue in the pulp chamber could be described as well-vascularized cell-rich connective tissue
The minimum diameter of the apical foramen to allow ingrowth is not determined, but a diameter much smaller than 1 mm does not prevent revascularization and ingrowth of new vital tissue (histologically).